Subscribe to Updates

    Get the latest creative news from FooBar about art, design and business.

    What's Hot

    4 Reasons Why Enterprises Should Consider Digital Transformation for Growth

    January 30, 2023

    Order your seafood online without worrying about quality

    January 27, 2023

    The Complete Ergonomic Desk Accessories Guide

    January 19, 2023
    Facebook Twitter Instagram
    • Home
    • Privacy Policy
    • Contact us
    Facebook Twitter Instagram Pinterest VKontakte
    ifvod.info ifvod.info
    • Home
    • News

      Order your seafood online without worrying about quality

      January 27, 2023

      The Complete Ergonomic Desk Accessories Guide

      January 19, 2023

      Guide For Tourists On Tessin | Travel Guide

      October 26, 2022

      Step-by-Step Home Loan Procedure That You Have to Go Through

      October 12, 2022

      What Does ISO 55001 Mean? And What Are The Benefits Of Certification?

      September 15, 2022
    • Entertainment
      1. Music
      2. View All

      How to Convert WAV to MP3 Files (and When You Should)

      November 30, 2021

      How to find the best speakers for classical music

      November 24, 2021

      Which are The best karaoke songs To dedicate your feelings this father’s day?

      November 24, 2021

      Learn indian classical music online – keyboard | Saamaveda music academy

      November 24, 2021

      5 Reasons People Love Hip-Hop Fitness Classes and Why You Should Teach It

      May 27, 2022

      3 Musical Styles On the Rise

      May 25, 2022

      BEST RAP SONGS OF 2022 SO FAR

      April 17, 2022

      Super Mario64: The best edition of nintendo

      November 24, 2021
    • Technology

      Make Rust a Thing of the Past with Laser Cleaning Machines for Metal

      December 29, 2022

      How to Develop an Effective Influencer Marketing Strategy

      November 22, 2022

      What is Valid IPTV?

      September 22, 2022

      how to stop someone from tracking your text messages

      August 15, 2022

      Know All The Things About Huawei Laptop Deals

      July 4, 2022
    • Fashion

      Custom Keychains: Which Styles Are the Best?

      December 25, 2022

      How To Find The Best High Waisted Cheeky Bikini

      November 11, 2022

      The Classic Shapes of Bags

      September 5, 2022

      Latest trends in Bridal jewellery

      July 26, 2022

      Sunflower T-Shirt Trend: Why Women Love the Flower

      July 25, 2022
    • Gaming

      Five Best Hunting Locations In Lost Ark

      December 15, 2022

      Everything You Need to Know About Teen Patti

      August 3, 2022

      How Warcraft Became The Most Powerful Game Franchise

      July 17, 2022

      Let’s Dive Into World Of สล็อตเว็บตรง

      June 15, 2022

      What Are the Major Reasons Behind APEX Legends Popularity?

      June 9, 2022
    • Lifestyle

      Hot New Fashion Trend: Sequin Suits

      October 21, 2022

      A Manual for Following To Tracking down BBW Dating

      June 23, 2022

      Drug Rehab, Florida Is The Best Place To Work On Yourself For Your Betterment

      June 10, 2022

      How to Get Free Instagram Followers in 2022

      June 8, 2022

      Breathable Shapewear You Can Wear in Summer

      June 3, 2022
    ifvod.info ifvod.info
    Home»News»Understanding The ELISA Method

    Understanding The ELISA Method

    SabithBy SabithMay 13, 2022No Comments4 Mins Read
    Share
    Facebook Twitter LinkedIn Pinterest Email

    The science and medicine world is high on the use of ELISA testing, it is something sort of revolutionary which has changed testing and is helping medicine further, testing be quicker, and the world become healthier. 

    Overall, you can theoretically get these tests with ease, check out MyBioSource ELISA Test Kits, for example. However, rather than telling you about that, we want to help you understand what the ELISA method really is.

    The Basic Principles

    ELISA (Enzyme-Linked Immunosorbent Assay) is a technique that is used in order to detect infectious agents and antibodies in a simple sample. 

    Antibodies are something that our body makes in response to infection, and so this type of ELISA testing can tell us if an animal has been in contact with a particular virus. Alternatively antigen ELISA tests can tell us if an animal is infected with a specific virus by detecting it.

    When using ELISA to test for an antibody, the antigens are stuck onto a surface made of plastic, and a sample is added to it, then any antibodies that are being tested will bind to these antigens. 

    Then another antibody with a particular marker will be added, and any positive reaction will be detected by this marker as it changes color when a particular substrate is included. If there are no antibodies, the second antibody won’t be able to stick and the color won’t change. 

    Alternatively for ELISA tests for antigens, the antibodies are bound to a surface made of plastic and a sample will be added. If antigens from the particular virus are there then they will stick to these antibodies. This will then go the same way as the ELISA antibody test.

    So, generally, it is actually a pretty simple process for testing.

    What Exactly IS An ELISA Test?

    ELISA is simply a test that will detect and measure any antibodies in your blood. It can be used to detect specific conditions. These antibodies are proteins that our bodies make in order to fight off antigens which are harmful to us.

    There are a few things ELISA is used to detect;

    • Lyme disease
    • HIV/ AIDS
    • Pernicious anemia
    • Rotavirus
    • Syphilis
    • Toxoplasmosis
    • Varicella-zoster virus (causes shingles and chickenpox) 
    • Squamous cell carcinoma
    • Rocky Mountain spotted fever
    • Zika virus

    ELISA is like the first call of action before more in-depth tests are used. Doctors can suggest ELISA if you have any signs or symptoms of an above condition, they may also use ELISA to rule out the above as well.

    How Are These Tests Done?

    These tests are actually really simple to do, even though they may sound somewhat complex at first. If you need an ELISA test done, you do need to sign a form and your doctor should explain why this test is being done. 

    ELISA means you will need to give a sample of your blood, this is not unlike any other time blood is taken, or even like giving blood. Your arm will be cleaned and then a band or tourniquet will be applied to your arm to create pressure and get your veins swelling. 

    A needle will be inserted into a vein to draw blood and when enough have been drawn the needle will be taken out, and you will receive a small bandage. 

    You may be asked to maintain pressure at this site for a little while to reduce the flow of blood, just like any other blood test. 

    It is pretty painless (unless you are squeamish), your arm might throb or feel heavy for a bit, but that’s about as bad as it gets. 

    Your sample will then be sent to the lab to be looked at, and the above tests will be done to test for whichever condition it is you have been tested for. The technician will run the tests, if the sample changes color you may have the condition, if not, you’re all clear! 

    What Are The Pros And Cons Of ELISA?

    Nothing is perfect as we know and while ELISA has plenty of upsides it does have downsides too. 

    Let’s consider the pros and cons of this fashion of testing.

    Pros

    • It is easy to do, 
    • It can determine the amount of antigen in a single sample. 
    • You can test various types of samples.
    • It is highly sensitive and specific. 
    • It is throughput, often available in 96-well plate, although assays can be adapted to 384- well plates.

    Cons

    • There is limited information on the antigen, it is limited to the amount in the sample.
    • The detection is based on substrate/enzyme reactions and so it’s a bit time restrictive.
    Sabith

    Related Posts

    Order your seafood online without worrying about quality

    January 27, 2023

    The Complete Ergonomic Desk Accessories Guide

    January 19, 2023

    Guide For Tourists On Tessin | Travel Guide

    October 26, 2022

    Step-by-Step Home Loan Procedure That You Have to Go Through

    October 12, 2022
    Add A Comment

    Leave A Reply Cancel Reply

    LATEST POSTS

    4 Reasons Why Enterprises Should Consider Digital Transformation for Growth

    January 30, 2023

    Order your seafood online without worrying about quality

    January 27, 2023

    The Complete Ergonomic Desk Accessories Guide

    January 19, 2023

    Make Rust a Thing of the Past with Laser Cleaning Machines for Metal

    December 29, 2022

    Custom Keychains: Which Styles Are the Best?

    December 25, 2022
    Categories
    • All
    • Business
    • Car
    • education
    • Entertainment
    • Fashion
    • Food
    • Gadget
    • Gaming
    • Health
    • Lifestyle
    • Music
    • News
    • Relationship
    • Sports
    • Technology
    ifvod.info
    Facebook Twitter Instagram Pinterest Vimeo YouTube
    • Home
    • Privacy Policy
    • Contact us
    © Copyright 2021, All Rights Reserved

    Type above and press Enter to search. Press Esc to cancel.

    Cleantalk Pixel